LOCUS       40924_35768       12483 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPZP 35S hRluc-attR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Subramanian C, Woo J, Cai X, Xu X, Servick S, Johnson CH, Nebenfuhr 
            A, von Arnim AG.
  TITLE     A suite of tools and application notes for in vivo protein 
            interaction assays using bioluminescence resonance energy transfer 
            (BRET)
  JOURNAL   Plant J. 48 (1), 138-152 (2006)
  PUBMED    16925598
REFERENCE   2  (bases 1 to 12483)
  AUTHORS   von Arnim AG, Subramanian C.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2005) BCMB, University of Tennessee Knoxville, 
            1406 Circle Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 12483)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12483)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2006"; volume: "48"; issue: "1"; pages: "138-152"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2005) BCMB, University of Tennessee Knoxville, 1406 Circle 
            Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1813
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2250..3314
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4247..4835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5082..5870)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    6398..6422
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6520..6694)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     6909..6925
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7030..7701
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             7851..8783
                     /label=hRluc
                     /note="Renilla luciferase"
     protein_bind    8790..8914
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        8939..8969
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             9023..9679
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             10024..10326
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(10370..10494)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      10565..10775
                     /label=35S cauliflower mosaic virus terminator
                     /note="35S cauliflower mosaic virus terminator"
                     /regulatory_class="terminator"
     promoter        10840..11220
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             11226..11774
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      11787..12039
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(12067..12083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12091..12107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12115..12145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12160..12181)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    12346..12370
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt aattcggggg atctataggg actttaggtg atctggattt tagtactgga
     6541 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
     6601 ctaagggttt cttatatgct caacacgtga gcgaaaccct ataagaaccc taattccctt
     6661 atctgggaac tactcacaca ttattatgga gaaactcgat gtcgatcgac tctagctaga
     6721 acgaattgtt aggtggcggt acttgggtcg atttggcaag ctgctctagc attcgccatt
     6781 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     6841 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     6901 acgacgttgt aaaacgacgg ccagtgccaa gcttgatatc gaattcctgc aggtcaacat
     6961 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     7021 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     7081 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     7141 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     7201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     7261 aaagcaagtg gattgatgtg ataacatggt ggagcacgac acacttgtct actccaaaaa
     7321 tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat
     7381 atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt
     7441 ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga
     7501 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     7561 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     7621 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     7681 ttcatttcat ttggagagga cctcgagaat tctcaacaca acatatacaa aacaaacgaa
     7741 tctcaagcaa tcaagcattc tacttctatt gcagcaattt aaatcatttc ttttaaagca
     7801 aaagcaattt tctgaaaatt ttcaccattt acgaacgata ggcctaggcc atggcttcga
     7861 aggtgtacga ccccgagcag aggaagagga tgatcaccgg cccccagtgg tgggccaggt
     7921 gcaagcagat gaacgtgctg gacagcttca tcaactacta cgacagcgag aagcacgccg
     7981 agaacgccgt gatcttcctg cacggcaacg ccgctagcag ctacctgtgg aggcacgtgg
     8041 tgccccacat cgagcccgtg gccaggtgca tcatccccga tctgatcggc atgggcaaga
     8101 gcggcaagag cggcaacggc agctacaggc tgctggacca ctacaagtac ctgaccgcct
     8161 ggttcgagct cctgaacctg cccaagaaga tcatcttcgt gggccacgac tggggcgcct
     8221 gcctggcctt ccactacagc tacgagcacc aggacaagat caaggccatc gtgcacgccg
     8281 agagcgtggt ggacgtgatc gagagctggg acgagtggcc agacatcgag gaggacatcg
     8341 ccctgatcaa gagcgaggag ggcgagaaga tggtgctgga gaacaacttc ttcgtggaga
     8401 ccatgctgcc cagcaagatc atgagaaagc tggagcccga ggagttcgcc gcctacctgg
     8461 agcccttcaa ggagaagggc gaggtgagaa gacccaccct gagctggccc agagagatcc
     8521 ccctggtgaa gggcggcaag cccgacgtgg tgcagatcgt gagaaactac aacgcctacc
     8581 tgagagccag cgacgacctg cccaagatgt tcatcgagag cgaccccggc ttcttcagca
     8641 acgccatcgt ggagggcgcc aagaagttcc ccaacaccga gttcgtgaag gtgaagggcc
     8701 tgcacttcag ccaggaggac gcccccgacg agatgggcaa gtacatcaag agcttcgtgg
     8761 agagagtgct gaagaacgag cagagatcta caagtttgta caaaaaagct gaacgagaaa
     8821 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     8881 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     8941 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt
     9001 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     9061 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     9121 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     9181 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     9241 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     9301 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     9361 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     9421 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     9481 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     9541 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     9601 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     9661 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     9721 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     9781 tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     9841 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     9901 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     9961 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt
    10021 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
    10081 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
    10141 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
    10201 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
    10261 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
    10321 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
    10381 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
    10441 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtcatgat
    10501 ctagagggaa gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
    10561 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
    10621 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
    10681 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
    10741 taaaatttct aattcctaaa accaaaatcc agtgacctgc agcccggggg atccactaga
    10801 gcggccgcca ccccgggtac cgagctcgaa ttctgagatt tttcaaatca gtgcgcaaga
    10861 cgtgacgtaa gtatctgagc tagtttttat ttttctacta atttggtcgt ttatttcggc
    10921 gtgtaggaca tggcaaccgg gcctgaattt cgcgggtatt ctgtttctat tccaactttt
    10981 tcttgatccg cagccattaa cgacttttga atagatacgc tgacacgcca agcctcgcta
    11041 gtcaaaagtg taccaaacaa cgctttacag caagaacgga atgcgcgtga cgctcgcggt
    11101 gacgccattt cgccttttca gaaatggata aatagccttg cttcctatta tatcttccca
    11161 aattaccaat acattacact agcatctgaa tttcataacc aatctcgata caccaaatcg
    11221 aatcgatgag cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc
    11281 cggcggtctg caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg
    11341 agccgcagga accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct
    11401 ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggc ccctggaagg
    11461 cacgcaacgc ctacgactgg acggccgagt cgaccgtgta cgtctccccc cgccaccagc
    11521 ggacgggact gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct
    11581 tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg
    11641 cgctcggata tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc
    11701 atgacgtggg tttctggcag ctggacttca gcctgccggt accgccccgt ccggtcctgc
    11761 ccgtcaccga gatctaactg cagaatcttg gactcccatg ttggcaaagg caaccaaaca
    11821 aacaatgaat gatccgctcc tgcatatggg gcggtttgag tatttcaact gccatttggg
    11881 ctgaattgaa gacatgctcc tgtcagaaat tccgtgatct tactcaatat tcagtaatct
    11941 cggccaatat cctaaatgtg cgtggcttta tctgtctttg tattgtttca tcaattcatg
    12001 taacgtttgc ttttcttatg aattttcaaa taaattatca gatccgagct cgaattcgta
    12061 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    12121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    12181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    12241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca gcttgagctt
    12301 ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg
    12361 gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga
    12421 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
    12481 caa
//
